Goats' milk xanthine oxidoreductase is grossly deficient in molybdenum

Xanthine oxidoreductase (XOR) was purified from goats' milk. The u.v.-visible absorption spectrum was essentially identical to those of the corresponding bovine and human milk enzymes and showed an A280/A450 ratio of 5.20+/-0.12, indicating a high degree of purity. Like bovine and human milk XORs, enzyme purified from goats' milk showed a single band on SDS-PAGE corresponding to a subunit with approximate Mr 150,000. On Western blotting, mouse monoclonal anti-human XOR antibody cross-reacted with purified caprine and bovine XORs. The specific xanthine oxidase activity of goats' milk XOR, however, was very much lower than that of bovine XOR, although NADH oxidase activities of XOR from the two sources were similar. In these respects, the caprine milk XOR mirrors the human milk enzyme, in which case the kinetic effects have previously been attributed to relatively low molybdenum content. The molybdenum content of goats' milk XOR also was shown to be relatively low, with 0.09 atoms Mo per subunit, compared with 055 atoms Mo per subunit for the bovine enzyme. A parallel purification of human milk XOR showed 0.03 atoms Mo per subunit. The possible physiological significance of the low molybdenum content of the caprine milk enzyme and of its correspondingly low enzymic activity is discussed.     

Frequency dispersion of electro-optical properties over a wide range by means of time-response analysis

We show that a Z-transform-based time-response analysis of the electro-optical response of a crystal to a step voltage with a short rise time allows one to obtain the dispersion of the electro-optical coefficients over a wide frequency range. We describe the method employed and present the results obtained for the main electroptic coefficients (r22, r61, and rc) of a standard LiNbO3 crystal. We also show that this method is able to provide even small values of the electro-optic coefficient as well as the dispersion within a wide frequency range, which is limited only by the rise time of the step voltage.     

Using bottom surface reflectance to map coastal marine areas: a new application method for Lyzenga's model

Remote sensing is widely used in coastal management. Lyzenga's model has been traditionally used to explain the relationship between bottom surface reflectance and the radiance level measured by satellite. Due to its central assumption, this model lacks accuracy compared with the other radiative transfer models. Nonetheless, it enables, with a single and simple equation, representation of the multiple optical processes taking place in coastal areas. Mapping processes associated with this model may include radiometric correction, a technique previously pointed out as a major driver of mapping accuracy. Radiometric correction is generally based on a depth-invariant index, efficient for clear waters (Jerlov water type I to II) but largely unsuitable when transparency decreases (Jerlov water type II to III). In order to overcome this problem, we developed a new index for radiometric correction, which combines bathymetry data with attenuation coefficients. The improved efficiency of our model with regard to the traditional depth invariant index was demonstrated through two case studies: Funakoshi Bay (Japan; Jerlov water type II) and the Gabes Gulf part located off Mahares (Tunisia; Jerlov water type II to III).     

An efficient heuristic for reliability design optimization problems

This paper develops an efficient heuristic to solve two typical combinatorial optimization problems frequently met when designing highly reliable systems. The first one is the redundancy allocation problem (RAP) of series-parallel binary-state systems. The design goal of the RAP is to select the optimal combination of elements and redundancy levels to maximize system reliability subject to the system budget and to the system weight. The second problem is the expansion-scheduling problem (ESP) of multi-state series-parallel systems. In this problem, the study period is divided into several stages. At each stage, the demand is represented as a piecewise cumulative load curve. During the system lifetime, the demand can increase and the total productivity may become insufficient to assume the demand. To increase the total system productivity, elements are added to the existing system. The objective in the ESP is to minimize the sum of costs of the investments over the study period while satisfying availability constraints at each stage. The heuristic approach developed to solve the RAP and the ESP is based on a combination of space partitioning, genetic algorithms (GA) and tabu search (TS). After dividing the search space into a set of disjoint subsets, this approach uses GA to select the subspaces, and applies TS to each selected subspace. Numerical results for the test problems from previous research are reported and compared. The results show the advantages of the proposed approach for solving both problems.     

Predicate Abstraction and Refinement for Model Checking VHDL State Machines

In this paper we present an automatic combination of abstraction-refinement by which we translate a VHDL model describing a state system to an initial equivalent abstract system described by SMV to explore its state space to verify CTL properties. We present the method implemented to compute automatically abstractions using decision procedures. This method can handle different kinds of infinite state systems including systems composed of concurrent components and it can be extended for more complex VHDL concepts. Abstract models may admit spurious counterexamples (false negative results) which are executions at the abstract level with no corresponding executions at the concrete level. We devise a new algorithm which analyzes such counterexamples and refine the abstract model correspondingly by eliminating gradually the false negative results. We illustrate our approach on an example and we confirm its effectiveness on a large design.     

Prototyping bio-nanorobots using molecular dynamics simulation and virtual reality

This paper presents a molecular mechanics study using a molecular dynamics software (NAMD) coupled to virtual reality (VR) techniques for intuitive bio-nanorobotic prototyping. Using simulated bio-nano environments in VR, the operator can design and characterize through physical simulation and 3D visualization the behavior of protein-based components and structures. The main novelty of the proposed simulations is based on the characterization of stiffness performances of passive joints-based proteins (α-helix deca-alanine, repressor of primer protein and immunoglobulin protein) and active joints-based viral protein motor (VPL) in their native environment. Their use as elementary bio-nanorobotic components are also simulated and the results discussed.     

Acoustic emission characterization of damage in short hemp‐fiber‐reinforced polypropylene composites

This article aims at investigating the effects of hygrothermal aging on the damage mechanisms of short white Hemp Fiber Reinforced Polypropylene (HFRP) composites with various fiber contents (10, 20, 30, and 40 wt%). Injected molded specimens were subjected to hygrothermal aging with a relative humidity of 80% and two temperatures, 25 and 50°C. The water absorption and its effect on tensile properties of HFRP composites were investigated. The Acoustic Emission (AE) technique combined with scanning electron microscopy observations was used to identify microstructural damage events leading to overall failure of the HFRP composites. This identification according to hemp‐fiber content and hygrothermal aging was made with an unsupervised method based on a statistical multi‐variable analysis (k‐means algorithm). The AE results indicate that the quality of fiber‐matrix interface plays a major role in the damage process of HFRP composites, shown by the number of AE signals induced by the interface failure and their amplitude ranges. POLYM. COMPOS. 37:1101–1112, 2016. © 2014 Society of Plastics Engineers     

Relationship between structure and rheological,mechanical and thermal properties of polylactide/Cloisite 30B nanocomposites

Melt‐state and solid state mechanical properties and thermal stability of polylactide layered silicate nanocomposites elaborated by melt intercalation were studied as a function of clay content. Wide angle X‐ray scattering results, transmission electron microscopy observations, and rheological measurements indicated that the clay was finely distributed in the polylactide matrix. Contrary to nonlinear mechanical properties, thermal and linear mechanical properties were shown to increase with increasing clay fraction. The nanoindentation measurements confirm the significant increase of linear mechanical properties previously observed by tensile tests. The good correlation of linear mechanical properties at the macrometric and nanometric scales is explained by the high dispersion degree of the nanofiller in the biodegradable polymer matrix. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010     

An alpha-tectorin gene defect causes a newly identified autosomal recessive form of sensorineural pre-lingual non-syndromic deafness, DFNB21

In our efforts to identify new loci responsible for non-syndromic autosomal recessive forms of deafness, DFNB loci, we have pursued the analysis of large consanguineous affected families living in geographically isolated areas. Here, we report on the study of a Lebanese family comprising nine members presenting with a pre-lingual severe to profound sensorineural isolated form of deafness. Linkage analysis led to the characterization of a new locus, DFNB21, which was assigned to chromosome 11q23-25. Already mapped to this chromosomal region was TECTA. This gene encodes alpha-tectorin, a 2155 amino acid protein which is a component of the tectorial membrane. This gene recently has been shown to be responsible for a dominant form of deafness, DFNA8/12. Sequence analysis of the TECTA gene in the DFNB21-affected family revealed a G to A transition in the donor splice site (GT) of intron 9, predicted to lead to a truncated protein of 971 amino acids. This establishes that alpha-tectorin mutations can be responsible for both dominant and recessive forms of deafness. Comparison of the phenotype of the DFNB21 heterozygous carriers with that of DFNA8/12-affected individuals supports the hypothesis that the TECTA mutations which cause the dominant form of deafness have a dominant-negative effect. The present results provide genetic evidence for alpha-tectorin forming homo- or heteromeric structures.